Phenolic Compound Production Increased In Vitro Regenerated Cichorium intybus L.

Chicory (Cichorium intybus L.) is a low-height perennial or biennial herb from the family of Asteraceae. Investigation different in vitro regeneration strategies Cichorium and increasing number secondary metabolites regenerated plant samples were aims research. Callus regenerations achieved basal growth media supplemented with regulators (PGRs). Whole was carried out by direct organogenesis leaf explant Murashige Skoog (MS) B5 naphthalene acetic (NAA) acid indole-3-butyric (IBA). The highest callus quantity produced MS medium indole-3-acetic (IAA) benzyl amino purine (BAP). combination concentrations PGRs used not only provided root shoot formation callus, but also caused change amounts phenolic components. In addition, some an increase compounds shoots developed explant. When plants that grow vivo are compared each other, it has been determined grown contain higher compounds. extracted ethanol/water (80:20 v/v). analysis (caftaric, chicoric, chlorogenic acids esculin) performed high-performance liquid chromatography (HPLC) inulin UV spectrophotometry. caftaric than vitro. Contrarily, esculin, chicoric samples. concentration valuable samples, especially tissue, gives hope for large-scale production under laboratory conditions.